| Vector |
Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: COL3A1
Genomic copy number: unique
Gene name: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type
IV, autosomal dominant)
Contains complete coding sequence?: N
Chromosome: 2; Localization: 2 q31-q32.3
Tissue: skin fibroblasts, neonatal (cell line JIMM-69)
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: EcoRI
Insert size (kb): 4.278
Cross references: DNA Seq. Acc.: X14420
Nucleotides 1-4278 of the insert correspond to
nucleotides 25-4303 of X14420. Insert lengths(kb): 4.277999877929688 Tissue: skin fibroblasts, neonatal (cell line JIMM-69) Gene product: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) [COL3A1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.4(doublet), 0.9; BglI-- 2.5, 1.4(doublet), 0.9(doublet), 0.52; EcoRI--4.6, 2.9; NcoI--4.7, 3.5; XhoI--3.9, 2.5, 1.1. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.25, 1.11; BglI--0.61, 1.94, 2.39, 3.28; EcoRI--4.27; NcoI--0.97, 4.07; PvuII--0.60, 0.84, 2.39, 3.09, 3.25, 4.13; SacI--0.96, 1.29, 2.08, 2.90; XhoI--1.05, 3.57. The insert contains 78 nt 5' untranslated sequence, and 4200 nt of the coding region. The insert ends at a naturally-occuring EcoRI site at nt 4303 of the sequence record. |
