ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2(doublet), 2.8, 2.2; HindIII--6.2, 2.15, 1.45, 1.05; SalI--11.5.
Contains S. cerevisiae GAL1 promoter region (from about 1 kb upstream of the GAL1 transcription start site to amino acid 33) fused to the bacterial lacZ gene.
West RW Jr., et al. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol. Cell. Biol. 4: 2467-2478, 1984. PubMed: 6392852
