| Insert |
DNA: cDNA Insert lengths(kb): 2.022000074386597 Tissue: fibroblast Gene product: procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55) [P4HB] |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI/HindIII--2.7, 1.5, 0.58; HindIII--4.9; SmaI--3.2, 1.6; XbaI--4.9; PstI--4.2, 0.6. Protein products from in vitro translation of RNA transcribed from pHTB linearized by HindIII are detected by monoclonal antibody J9. The molecular weights of these in vitro products are 57, 53, and 45 kDa. Insert includes a PstI site at 509; AvaI sites at 133, 979, 1525, 1617, and 1637; a BamHI site at 1801, and a HindIII site at 2022. Insert includes 5' untranslated sequences, a full coding sequence, and a truncated 3' untranslated sequence. Hybridizes with a 2.7 kb mRNA in human A431, KB, HepG2, WI38, and rat GH3 cell lines. A minor mRNA of 4.9 kb is detected in KB, HepG2, and WI38. Constructed by subcloning the 1.5 kb PstI/HindIII and the 0.5 kb PstI/PstI fragments from p5A5 (ATCC 59480/59481) into pGEM3. |
| References |
Popescu NC, et al. Chromosomal localization of the gene for human thyroid hormone-binding protein. Am. J. Hum. Genet. 42: 560-564, 1988. PubMed: 2831713
Cheng SY, et al. The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum. J. Biol. Chem. 262: 11221-11227, 1987. PubMed: 3611107
Sheue-Yann Cheng, personal communication
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