transformation host permitting specialized production processes
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Shipping Information
Distributed: freeze-dried
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Preceptrol®
no
Genotype
thi- ppc-
Type Strain
no
Comments
NOTE: The deposited strain does NOT conform to the expected phenotype. It is able to grow on media lacking succinate.
This host allows control of cell mass while producing a biosynthetic compound via fermentation when transformed with a suitable plasmid.
It is best suited for the production of compounds which do not yield a net increase in TCA cycle intermediates, are not products of the glycolytic pathway, and are biosynthetically derived solely from glycolytic intermediates.
Derived from the strain YMC9 (ATCC 33927) by mud(amp lac)1 insertional mutagenesis and then removal of the beta-lactam resistance.
Recommendation for verification: Ability to grow on M9 media + 2 mg/ml glucose + 1 ug/ml vitamin B1 + 0.5 mg/ml succinate. Inability to grow on M9 media + 2 mg/ml glucose + 1 ug/ml vitamin B1.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor
Biotechnica International, Inc., KC Backman, Biotechnica International, Inc.
