| Comments |
Insert contains the following sites separated by (bp): KpnI- 380- PstI- 125- SacI- 495- PstI- 155- SmaI- 285- SmaI- 200- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; KpnI--5.0; EcoRI/KpnI--3.1, 1.8; SacI--5.0; PstI--3.7, 0.7, 0.5. Contains a 1296 nt open reading frame (starting at position 317) that encodes a 432 aa protein. RNA can be transcribed in vitro from vector T7 promoter. This appears to be a retinoic acid-inducible, trans-acting enhancer factor. Enzyme(s) not detecting polymorphism: ApaI, ApaLI, BamHI, BanI, BanII, BglII, BstNI, BxtXI, DraI, EcoO109, EcoRI, HindIII, MspI, NheI, PvuII, ScaI, StuI, TaqI, Tth111I, XmnI (in a minimum of 10 independent chromosomes). |
| References |
Mattei MG, et al. Mapping of the human retinoic acid receptor to the q21 band of chromosome 17. Hum. Genet. 80: 186-188, 1988. PubMed: 2844649
Zelent A, et al. Cloning of murine alpha and beta retinoic acid receptors and a novel receptor gamma predominantly expressed in skin. Nature 339: 714-717, 1989. PubMed: 2544807
Arveiler B, et al. A PstI RFLP for the human retinoic acid receptor in 17q21. Nucleic Acids Res. 16: 6252, 1988. PubMed: 2899875
Petkovich M, et al. A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature 330: 444-450, 1987. PubMed: 2825025
Pierre Chambon, personal communication
|
