| Vector |
Construct size (kb): 42.70000076293945 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaFIX
Intact vector size: 41.900
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: SalI XhoI EcoRI
Polylinker sites: XbaI SacI NotI SacI SalI XhoI EcoRI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: T3, T7
replicon: lambda
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC6A5
Other gene symbols: NET1
Gene name: solute carrier family 6 (neurotransmitter transporter,
norepinephrine), member 5
ORF's seq. position: GDB probe: GDB:136379 ()
Contains complete coding sequence?: N
Chromosome: 16; Localization: 16 q13-q21
Tissue: lung fibroblast
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 13.5
Cross references: DNA Seq. Acc.: X91118
Nucleotides ~7390~8410 of the insert correspond to
nucleotides 1-1017 of X91118.
DNA Seq. Acc.: X91119
Nucleotides ~9600~10400 of the insert correspond to
nucleotides 1-922 of X91119.
DNA Seq. Acc.: X91124
Nucleotides ~1700~2060 of the insert correspond to
nucleotides 1-357 of X91124.
DNA Seq. Acc.: X91125
Nucleotides ~3110~3520 of the insert correspond to
nucleotides 1-361 of X91125.
DNA Seq. Acc.: X91126
Nucleotides ~4060~4460 of the insert correspond to
nucleotides 1-395 of X91126.
DNA Seq. Acc.: X91127
Nucleotides ~5780~6760 of the insert correspond to
nucleotides 1-980 of X91127. Insert lengths(kb): 13.5 Tissue: lung fibroblast Gene product: solute carrier family 6 (neurotransmitter transporter, norepinephrine), member 5 [NET1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): NotI-- >9.0; EcoRI-- >9.0, 5.2, 0.6, 0.5; SacI-- >9.0, 4.4, 3.2, 2.6, 2.0, 1.7, 0.25; XbaI-- >9.0, 4.0, 1.2. The insert contains the following restriction sites (approximate kb from 5' end): EcoRI--1.30, 1.90, 2.40, 7.60; SacI--3.35, 7.75, 7.95, 9.90, 13.05; XbaI--9.55, 13.45. ATCC 99548 contains exons 6-14 of the 14 exons which comprise the norepinephrine transporter gene. |
