Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Rana pipiens, Anaoka County, MN, 1950
Product Format
frozen
Type Strain
no
Comments
Phylogenetic relationships among anuran trypanosomes
Species compared using RAPDs
Karyotype analysis of anuran trypanosomes
Medium
ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor
LS Diamond
Year of Origin
1950
References
Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144
Lun ZR, Desser SS. Analysis of isolates within species of anuran trypanosomes using random amplified polymorphic DNA. Parasitol. Res. 82: 22-27, 1996. PubMed: 8825440
Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044
