| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--8.5, 5.2 (doublet), 4.3, 3.8, 3.5, 2.7, 2.3 (doublet), 1.8 (triplet), and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 0.798, 0.427, 1.892, 5.204, 1.779, 0.560, 2.265, 1.586, 0.554, 3.361, 2.745, 3.650, 0.824, 5.020, 2.282, 1.784, 4.161. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70056, 70225, 70263, 70775. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
