Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Type Strain
no
Comments
Preservation
Life cycle
Medium
ATCC® Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Subcultivation
Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Name of Depositor
DL Wise
Chain of Custody
ATCC <<--DL Wise<<--UTEX 963 <<--- E.G. Pringsheim -- (?)
References
Reed RB, et al. Preservation of encysted Polytomella. J. Gen. Microbiol. 97: 29-34, 1976. PubMed: 186558
Lewis E, et al. Life cycle of Polytomella caeca (phytomonadida, Polyblepharidae). J. Protozool. 21: 647-649, 1974.
