| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--10.5, 7.0 (triplet), 6.2, 3.5, 2.4, 2.05, 0.98, 0.90, 0.82, 0.68; BglII--8.6, 6.6, 5.8, 5.3, 4.4, 3.3, 3.15, 2.95, 2.3 (doublet), 1.9, 0.54, 0.3; EcoRI--10.0, 7.0 (doublet), 6.2 (doublet), 4.1, 2.9, 2.4, 1.7, 1.1; HindIII--15.0, 11.0, 5.8, 5.6, 4.3, 3.3, 1.8, 1.05, 0.7, 0.48; NotI--uncut; SstI--15.5, 13.0, 10.5, 8.0, 2.3, 0.94, 0.67. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins 15038 nt upstream of exon 1 and continues past exon 2' into intron 3'. The upstream region contains a TATA box, several protein binding sites, a repeat region (13983-14521) and an inverted repeat (16054-16330). |
| References |
Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859
Heisterkamp N, et al. The first BCR gene intron contains breakpoints in Philadelphia chromosome positive leukemia. Nucleic Acids Res. 16: 10069-10081, 1988. PubMed: 3194194
Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185
Bruce A Roe, personal communication
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