| Insert |
DNA: cDNA Insert lengths(kb): 1.759999990463257 Tissue: liver Gene product: cytochrome P450, subfamily IIA (phenobarbital-inducible)(coumarin 7-hydroxylase) [CYP1] Alleles: A1, A1, A2, A2, A1, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): AccI--4.5; XhoI--uncut; HindIII--4.5; EcoRI--2.8, 1.75; KpnI--4.5. CYP2A (pMP12) and CYP2B (pMP17) hybridize to the same MluI (350 kb), NotI (520 kb), and SstII (350 kb) fragments, but to different BssHII, NaeI, NarI, SfiI, and SmaI fragments. This probe detects the SstI (SacI) RFLP originally reported by Wainwright. Three sizes of transcripts (1.9 kb, 2.7 kb, and 3.8 kb) are identified in Northern blots from human liver, with the 1.9 kb transcript being most prevalent. Each transcript demonstrated significant inter-individual variation (n=6). Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, MspI, PstI, PvuII, RsaI, TaqI and XmnI. |
| References |
Miles JS, et al. Close linkage of the human cytochrome P450IIA and P450IIB gene subfamilies: implications for the assignment of substrate specificity. Nucleic Acids Res. 17: 2907-2917, 1989. PubMed: 2726448
Wainwright BJ, et al. RFLP for a human cytochrome P-450 gene at 19q13.1-qter (HGM8 provisional designation CYP1). Nucleic Acids Res. 13: 4610, 1985. PubMed: 4011450
John S Miles, personal communication
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