Restriction digests of the clone give the following sizes (kb): HindIII/NotI--5.4, 2.5; BamHI--3.0, 2.85, 2.1; PstI--6.4, 1.5; SmaI--5.2, 2.75; EcoRI--4.4, 3.5. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.08, XbaI--0.33; SfiI--1.17; SmaI--1.33; SacI--1.34, 1.57, 2.25. Contains the complete coding sequence (nt 180-1748) plus 5' and 3' untranslated regions. The 5' end of the insert is adjacent to the unique HindIII and SpeI sites of the MCS; the 3' end of the insert is adjacent to the unique NotI site of the MCS. |
